We unveil and characterize a new N-methyltransferase (NMT) enzyme from the plant T. elegans: perivine Nβ-methyltransferase (TePeNMT). Enzyme kinetic experiments and enzyme homology modeling studies reveal the significant differences in enzyme active sites between TePeNMT and CrPeNMT, a previously characterized perivine NMT from C. roseus.
Collectively, our findings suggest that parallel evolution of ancestral γTMTs may be responsible for the occurrence of perivine N-methylation in T. elegans and C. roseus
https://doi.org/10.3389/fpls.2024.1451298
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